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olga:olgappraseptequ [2019-06-13 21:55]
danield 		olga:olgappraseptequ [2020-06-25 19:54] (current)
danield
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-Hier findet ihr eine gute Zusammenfassung der wichtigen Grundlagen für steriles ​arbeiten ​im mikrobiologischem ​Labor. Diese Techniken sollte man sich vorher zur Genüge tun um sich und seine Experimente vor ungewollten Kontaminationen (Verunreinigungen) zu schützen. Mit diesen Tipps seid ihr ausreichend informiert um selbst im Labor aktiv zu werden :) +Hier findet ihr eine gute Zusammenfassung der wichtigen Grundlagen für steriles ​Arbeiten ​im mikrobiologischen ​Labor. Diese Techniken sollte man berücksichtigen, ​um sich und seine Experimente vor ungewollten Kontaminationen (Verunreinigungen) zu schützen. Mit diesen Tipps seid ihr ausreichend informiert um selbst im Labor aktiv zu werden :) 
-Der Text stammt von Microbiology Notes.+Der Text stammt von [[https://​microbenotes.com/​general-aseptic-techniques-in-microbiology-laboratory/​|Microbiology Notes]].
  
-======General Aseptic Techniques in Microbiology Laboratory======+====== General Aseptic Techniques in Microbiology Laboratory ======
  
 - Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms (i.e., sepsis). - Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms (i.e., sepsis).
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 - The aseptic techniques control the opportunities for contamination of cultures by microorganisms from the environment,​ or contamination of the environment by the microorganisms being handled. - The aseptic techniques control the opportunities for contamination of cultures by microorganisms from the environment,​ or contamination of the environment by the microorganisms being handled.
  
 +----------------
  
-__Examples ​of aseptic technique are:__+ 
 +**Examples ​of aseptic technique are:**
  
 - Cleaning and disinfecting lab surfaces prior to use - Cleaning and disinfecting lab surfaces prior to use
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 - avoiding breathing on cultures or sterile instruments. - avoiding breathing on cultures or sterile instruments.
 +
 +
 +----------------
  
  
  
-*There are some general rules to follow for any aseptic technique.*+**There are some general rules to follow for any aseptic technique.**
  
 - Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air. - Close windows and doors to reduce draughts and prevent sudden movements which might disturb the air.
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-*Specific Aseptic Techniques*+----------------
  
-Sterile Handling+ 
 + 
 +===== Specific Aseptic Techniques ===== 
 + 
 + 
 +**Sterile Handling**
  
 - Always wipe your hands and work area with 70% ethanol. - Always wipe your hands and work area with 70% ethanol.
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-*Inoculating agar plates, slopes and cultures*+---------------- 
 + 
 + 
 +**Inoculating agar plates, slopes and cultures**
  
 - Carry out the transfer of cultures as quickly as possible, with tubes and plates open to the air for the minimum length of time. - Carry out the transfer of cultures as quickly as possible, with tubes and plates open to the air for the minimum length of time.
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-*Using a wire loop*+---------------- 
 + 
 + 
 + 
 +**Using a wire loop**
  
 - While using a wire loop, hold the handle of the wire loop close to the top, as you would hold a pen, at an angle that is almost vertical. This leaves the little finger free to take hold of the screw cap/ cotton wool plug of the bottle/ test tube. It also ensures that any liquid culture on the loop will run down into the flame. - While using a wire loop, hold the handle of the wire loop close to the top, as you would hold a pen, at an angle that is almost vertical. This leaves the little finger free to take hold of the screw cap/ cotton wool plug of the bottle/ test tube. It also ensures that any liquid culture on the loop will run down into the flame.
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-*Using a pipette*+---------------- 
 + 
 + 
 + 
 +**Using a pipette**
  
 - Sterile graduated or dropping (Pasteur) pipettes are used to transfer cultures, sterile media and sterile solutions. - Sterile graduated or dropping (Pasteur) pipettes are used to transfer cultures, sterile media and sterile solutions.
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-*Flaming the neck of bottles and test tubes*+---------------- 
 + 
 + 
 + 
 +**Flaming the neck of bottles and test tubes**
  
 This ensures that no microorganisms enter the mouth of the vessel to contaminate the culture or the medium. Passing the mouth of the bottle through a flame produces a convection current away from the opening, and helps to prevent contamination. The hot part of the flame is above the inner bright blue ‘cone’ and the vessel needs to be moved through the flame, not held in place. This ensures that no microorganisms enter the mouth of the vessel to contaminate the culture or the medium. Passing the mouth of the bottle through a flame produces a convection current away from the opening, and helps to prevent contamination. The hot part of the flame is above the inner bright blue ‘cone’ and the vessel needs to be moved through the flame, not held in place.
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-*The Bunsen Burner*+---------------- 
 + 
 + 
 + 
 +**The Bunsen Burner**
  
 - Probably the easiest way to create a relatively sterile environment on the laboratory bench is by using a simple gaspowered burner. - Probably the easiest way to create a relatively sterile environment on the laboratory bench is by using a simple gaspowered burner.
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-*The Laminar Flow Unit*+ 
 +---------------- 
 + 
 + 
 +**The Laminar Flow Unit**
  
 - A laminar flow unit (or hood) is a sophisticated appliance that can further help prevent contamination of reagents and biological cultures. - A laminar flow unit (or hood) is a sophisticated appliance that can further help prevent contamination of reagents and biological cultures.
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-*References*+---------------- 
 + 
 + 
 +**References**
  
 1) pbf.unizg.hr/​content/​download/​24827/​96881/​version/​…/​Aseptic+Technique.pdf 1) pbf.unizg.hr/​content/​download/​24827/​96881/​version/​…/​Aseptic+Technique.pdf
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