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olga:olgainventarchemikalien [2020-01-09 19:56] danield [Kühl- & Gefrierschrank (Lagerung bei 4°bzw. -20°C)] |
olga:olgainventarchemikalien [2020-09-09 21:17] danield [Nährmedium (Mikrobiologie)] |
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Line 23: | Line 23: | ||
**G** | **G** | ||
- | |Gluconsäure|Gylcerin|Glycin| | + | |Gluconsäure|Gylcerol=Glycerin|Glycin| |
**H** | **H** | ||
Line 68: | Line 68: | ||
====Nährmedium (Mikrobiologie)==== | ====Nährmedium (Mikrobiologie)==== | ||
- | **In Gebinde:** | + | |Agar-Agar|Chloramphenicol|[[olga:citratmedium|Citrat Medium]]|| |
+ | |Hefeextrakt|LB Medium|[[olga:lbvib|LB (Vibrio) Medium]]| | ||
+ | |MacConkey-Agar|Malzextrakt|[[olga:meamed|MEA Medium]] | ||
+ | |Nährbouillon|Pepton|MRS| | ||
+ | |Rindsbrühe bzw. Suppe|Streptomycinsulfat|TBX-Chromo-Agar| | ||
+ | |Terrific Broth|Trypton|YPD| | ||
+ | |Blut Agar (Basis)|Erd-Wasser Medium|[[https://realraum.at/wiki/lib/exe/fetch.php?media=olga:algea_culture_media_m343.pdf|Algen Medium]]| | ||
+ | |DEV Tryptophan-Bouillon| | ||
+ | |Clostridien-Differential-Bouillon| | ||
+ | |m-CP-Agar (Basis)| | ||
+ | ====DNA-/Plasmid Minipräp Kit==== | ||
+ | inklusive Protokoll | ||
- | |Agar-Agar|Chloramphenicol|Glukose 50%| | + | Lösung 1 |
- | |Hefeextrakt|MacConkey-Agar|Malzextrakt| | + | |50 mM Tris/HCl pH=8|10 mM EDTA pH=8|RNAse A 100µg/mL| |
- | |Nährbouillon|Pepton|Riboflavin + Zucker|Rindsbrühe bzw. Suppe| | + | |
- | |Streptomycinsulfat|TBX-Chromo-Agar|Terrific Broth| | + | |
- | |Trypton|YPD| | + | |
- | + | ||
+ | Lösung 2 | ||
+ | |0,2 M NaOH| 1% (w/v) SDS| | ||
- | **"Mission is possible:" Selbst mischbare Medien** | + | Lösung 3 |
+ | |2,8 M KAc, pH=5,1| | ||
+ | Isopropanol 99% | ||
- | |__LB Medium__|__LB (Vibrio)__|__[[olga:citratmedium|Citrat Medium]]__|__[[https://realraum.at/wiki/lib/exe/fetch.php?media=olga:algea_culture_media_m343.pdf|Algen Medium]]__| | + | Ethanol 70 % |
- | |Hefeextrakt 5 g|LB mit 3% NaCl|Diammoniumsulfat 0,22 g| | + | |
- | |Trypton 10 g|mit ddH20 auf 1 L|Kaliumdihydrogenphosphat 1,824 g| | + | |
- | |NaCl 1-10 g| |Magnesiumchlorid 0,158 g| | + | |
- | |mit ddH20 auf 1 L||Bromthymolblau 80 mg| | + | |
- | | | |NaCl 4,806 g| | + | |
- | | | |Zitronensäure 1,489 g| | + | |
- | | | |NaOH 1,063 g| | + | |
- | | | |KOH 41 mg| | + | |
- | | | |Ammoniak (25%) 0,407 mL| | + | |
- | | | |mit ddH20 auf 1 L| | + | |
- | + | ||
- | * für **Agar Platten 1,5% Agar Agar** zugeben (Volumen ca. 1/3 von Gefäß) | + | |
- | + | ||
- | * bei 121°C für mind. 20 min autoklavieren (bei Agar einen Rührknochen vorher hinzu geben) | + | |
Line 112: | Line 109: | ||
===Kühlschrank (4°C):=== | ===Kühlschrank (4°C):=== | ||
- | Was|Wo | + | |WAS|WO| |
+ | |||
+ | |Bakteriophagen (origin. & Lysate)| Tür links oben| | ||
+ | |Eurofin Genetics Primer| Tür rechts oben| | ||
+ | |Nippon Green Genetics|Tür Antibiotika Stock| | ||
+ | |Penicillin 5 mg/mL|Tür Antibiotika Stock| | ||
+ | |Proteinase K|Tür Antibiotika Stock| | ||
+ | |Ampicillin Natriumsaltz|Tür Antibiotika Stock| | ||
+ | |Kanamycinsulfat|Tür Antibiotika Stock| | ||
+ | |1,4-Dithiothreit|Tür Antibiotika Stock| | ||
+ | |Geneticinsulfat|Tür Antibiotika Stock| | ||
+ | |DIY Lysozym|Tür Antibiotika Stock| | ||
+ | |diverse Agarplatten Reserve| Tür unten| | ||
+ | | | ||
+ | |Agarplatten mit Mikroorganismen| aufgeteilt im Kühlschrank| | ||
+ | |||
+ | |||
+ | Gefrierfach im Kühlschrank: | ||
+ | 3x Kühlelemente (BIOLabs) | ||
+ | 1x blaues Kühlelement für Eppis | ||
+ | |||
+ | |||
+ | ------------------------------ | ||
===Gefrierschrank (-20°C):=== | ===Gefrierschrank (-20°C):=== | ||
+ | |||
+ | **Fach OBEN** | ||
+ | |||
+ | __Weiß1 = Bufferbox__ | ||
+ | |||
+ | NEBuffer2.1 |||| | ||
+ | |||
+ | NeBuffer3.1 | | ||
+ | |||
+ | CutSmartBuffer ||||| |||| | ||
+ | |||
+ | OneTaqStandardReactionBuffer |||| | ||
+ | |||
+ | OneTagGCReactionBuffer | | ||
+ | |||
+ | OneTaqHighGCEnhancer | | ||
+ | |||
+ | Buffer f. T4 DNA Ligase+ATP |||| | ||
+ | |||
+ | Buffer f. T4 DNA Ligase | | ||
+ | |||
+ | Buffer f. T7 DNA Ligase | | ||
+ | |||
+ | TermoPolReactionBuffer ||||| | ||
+ | |||
+ | ThermoPolBuffer || | ||
+ | |||
+ | ATP20xStock (Ligation) | | ||
+ | |||
+ | 5x HF Puffer | | ||
+ | |||
+ | Magnesiumchlorid | ||
+ | |||
+ | __Weiß2 = Primer__ | ||
+ | |||
+ | Primer reverse | | ||
+ | |||
+ | Primer forward | | ||
+ | |||
+ | Primer mix | | ||
+ | |||
+ | Primer forward (Eurofins) | ||
+ | |||
+ | Controll Template | ||
+ | |||
+ | Controll Primer || | ||
+ | |||
+ | p15a forward primer | ||
+ | |||
+ | LacZ PRimer forward | | ||
+ | |||
+ | NDE1 reverse | | ||
+ | |||
+ | Kpn1 forward | | ||
+ | |||
+ | |||
+ | |||
+ | dNTP-SolutionMix (10mM) || | ||
+ | |||
+ | dNTP (2mM) ||||||||||||| | ||
+ | |||
+ | |||
+ | |||
+ | Lamda-DNA | | ||
+ | |||
+ | Lamda DNA Hind3 digest | | ||
+ | |||
+ | Quick-(1kB) DNA Ladder | | ||
+ | |||
+ | 100bp DNA Ladder | | ||
+ | |||
+ | |||
+ | |||
+ | Controll Template ||| | ||
+ | |||
+ | Controll Primer ||| | ||
+ | |||
+ | |||
+ | |||
+ | pUC192 | | ||
+ | |||
+ | pUC19 neu | | ||
+ | |||
+ | pEasy T1 Cloning Vector | | ||
+ | |||
+ | pYAC3 || | ||
+ | |||
+ | pGreen20049 | | ||
+ | |||
+ | p355-GFP | | ||
+ | |||
+ | |||
+ | |||
+ | CUP2 Promotor|| | ||
+ | |||
+ | SPO3 forward Primer | | ||
+ | |||
+ | Gene Ruler 100bp | | ||
+ | |||
+ | |||
+ | __Grün 3 = Polymerase/RNase__ | ||
+ | |||
+ | |||
+ | TE + RNase ||||||||||| | ||
+ | |||
+ | Taq Polymerase ||||| | ||
+ | |||
+ | Phusion HF Dna Pol | | ||
+ | |||
+ | RNase-IF | | ||
+ | |||
+ | RNase|||| | ||
+ | |||
+ | Instant Sticky Ends Ligase Master Mix | | ||
+ | |||
+ | T4 Dna Ligase | | ||
+ | |||
+ | T7 Dna Ligase | | ||
+ | |||
+ | Blunt Ligation Master Mix | | ||
+ | |||
+ | Taq Buffer | ||
+ | |||
+ | |||
+ | |||
+ | __Blau4__ | ||
+ | |||
+ | |||
+ | 5x Phusion GC Reaction Buffer | | ||
+ | |||
+ | Q5 Reaction Buffer || | ||
+ | |||
+ | Q5 Hf Polymerase || | ||
+ | |||
+ | 1kb + DNA Ladder | | ||
+ | |||
+ | Eurofins Primer | | ||
+ | |||
+ | Phusion HF Reaction Buffer | | ||
+ | |||
+ | 10mM dNTP Solution Mix ||| | ||
+ | |||
+ | Q5 High GC Enhancer || | ||
+ | |||
+ | OneTaq Dna Pol | | ||
+ | |||
+ | OneTaq HotStart Buffer | | ||
+ | |||
+ | 10 kb controll Primer Mix | | ||
+ | |||
+ | 100% DMSO | | ||
+ | |||
+ | |||
+ | __Box 5 - blauer Deckel__ | ||
+ | |||
+ | Lamda DNA | | ||
+ | |||
+ | Buffer | ||
+ | |||
+ | Eurogenomics Primer | ||
+ | |||
+ | Primer | ||
+ | |||
+ | LycTerm | ||
+ | |||
+ | DNA Marker Quick Load | | ||
+ | |||
+ | Loading Buffer | ||
+ | |||
+ | 100bP Dna Ladder | ||
+ | |||
+ | One Taq Standard Reaction Buffer| | ||
+ | |||
+ | |||
+ | __Metallblock mit Restriktionsenzyme__ | ||
+ | |||
+ | Sp1 | | ||
+ | |||
+ | Bsa!-HF || | ||
+ | |||
+ | Pst1 | | ||
+ | |||
+ | Kq/pn1 | ?? | ||
+ | |||
+ | Xma1 | | ||
+ | |||
+ | BamH1 -HF| | ||
+ | |||
+ | Xba1 | | ||
+ | |||
+ | Sma1| | ||
+ | |||
+ | EcoR1-HF| | ||
+ | |||
+ | PNK | | ||
+ | |||
+ | Pst1 | | ||
+ | |||
+ | T4 Dna Ligase ||| | ||
+ | |||
+ | Proteinase K | | ||
+ | |||
+ | T4 DNA Ligase + ATP || | ||
+ | |||
+ | |||
+ | |||
+ | __Violette Box (to be used)__ | ||
+ | |||
+ | |||
+ | Ribunuklease 100mg | | ||
+ | |||
+ | Iptg 1g | | ||
+ | |||
+ | HOT FIREPOL Blend Mastermix | | ||
+ | |||
+ | xß-gal | | ||
+ | |||
+ | Lyticase | | ||
+ | |||
+ | GFP | ||
+ | |||
+ | ATP 5 g | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | __Phusion High fidelity PCR Kit__ | ||
+ | |||
+ | |||
+ | Phusion DNA Pol | | ||
+ | |||
+ | 1.3 kB Primer | | ||
+ | |||
+ | GC Buffer| | ||
+ | |||
+ | DMSO | | ||
+ | |||
+ | 10 kb Primer | | ||
+ | |||
+ | Magnesiumchlorid | | ||
+ | |||
+ | dNTP Mix | | ||
+ | |||
+ | DNA-Marker| | ||
+ | |||
+ | Lamda-DNA | | ||
+ | |||
+ | |||
+ | |||
+ | __E.coli Selection Kit__ | ||
+ | |||
+ | versciedene Vektoren, Buffer, PCR-Controll, Restrictionsenzyme... | ||
+ | |||
+ | |||
+ | __STÄMME (lyophilisiert in Plastikröhrchen__ | ||
+ | |||
+ | DSM430 | | ||
+ | |||
+ | DSM5997 | | ||
+ | |||
+ | __Eurofins Genomix Metall__ | ||
+ | |||
+ | |||
+ | Primer |||| | ||
+ | |||
+ | |||
+ | __NAWI - iGem Plastik Hülsn__ | ||
+ | |||
+ | |||
+ | G-Blocks Gen Fragmente | | ||
+ | |||
+ | Spezifisch-Flag-Tag | | ||
+ | |||
+ | |||
+ | __Transformation Efficiency Kit - Plasik Hülsn__ | ||
+ | |||
+ | |||
+ | pSB1C3 50pg/ml - 0,5pg/ml) | ||
+ | |||
+ | |||
+ | __Linearisirte Plasmid Backbones - Plastik Hülsn__ | ||
+ | |||
+ | pSB1 derivate 4x | ||
+ | |||
+ | |||
+ | |||
+ | **Fach MITTE** | ||
+ | |||
+ | Transgen Biotech - China Free Samples !!! Protokolle zu machnen Enzymen aus Fach OBEN | ||
+ | |||
+ | |||
+ | **Fach UNTEN** | ||
+ | |||
+ | Unspezifische DNA (irgendwelche DNA Proben ausn Praktikum, verwenden) | ||
+ | |||
+ | Spezifische DNA (Bekannte DNA Proben, Plasmide, usw) | ||
+ | |||
+ | Gelladepuffer (6x) | ||
+ | |||
+ | Antibiotika Stock Lösungen ( ready to use --> für 100 mL Medium = 100 µL Stock-Lsg) | ||
+ | -Ampicillin | ||
+ | -Kanamycin | ||
+ | -Streptomycin | ||
+ | -Chloramphenicol | ||
+ | |||
+ | Molbio Free 4 All Box (diverse Reagenzien) | ||
+ | |||
+ | Daniel Derndorfer Box (privat Box --> nix ohne fragen herausnehmen, bitte :) ) | ||
+ | |||
+ |
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