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olga:olgainventarchemikalien [2020-01-09 19:54]
danield
olga:olgainventarchemikalien [2020-04-09 23:37]
danield
Line 23: Line 23:
  
 **G** **G**
-|Gluconsäure|Gylcerin|Glycin|+|Gluconsäure|Gylcerol=Glycerin|Glycin|
  
 **H** **H**
Line 68: Line 68:
 ====Nährmedium (Mikrobiologie)==== ====Nährmedium (Mikrobiologie)====
  
-**In Gebinde:​**  +|Agar-Agar|Chloramphenicol|[[olga:​citratmedium|Citrat Medium]]|
- +|Hefeextrakt|LB Medium|LB (Vibrio)| 
-|Agar|Chloramphenicol|Glukose 50%+|MacConkey-Agar|Malzextrakt| 
-|Hefeextrakt|MacConkey-Agar|Malzextrakt| +|Nährbouillon|Pepton|Riboflavin + Zucker
-|Nährbouillon|Pepton|Riboflavin + Zucker|Rindsbrühe bzw. Suppe+|Rindsbrühe bzw. Suppe|Streptomycinsulfat|TBX-Chromo-Agar
-|Streptomycinsulfat|TBX-Chromo-Agar|Terrific Broth+|Terrific Broth|Trypton|YPD|
-|Trypton|YPD|+
   
  
  
-**"​Mission is possible:"​ Selbst mischbare Medien**+====DNA-/​Plasmid Minipräp Kit==== 
 +inklusive Protokoll
  
 +Lösung 1
 +|50 mM Tris/HCl pH=8|10 mM EDTA pH=8|RNAse A 100µg/​mL| ​
  
-|__LB Medium__|__LB (Vibrio)__|__[[olga:​citratmedium|Citrat Medium]]__|__[[https://​realraum.at/​wiki/​lib/​exe/​fetch.php?​media=olga:​algea_culture_media_m343.pdf|Algen Medium]]__| +Lösung 2 
-|Hefeextrakt 5 g|LB mit 3% NaCl|Diammoniumsulfat ​0,22 g| +|0,2 M NaOH| 1(w/vSDS|
-|Trypton 10 g|mit ddH20 auf L|Kaliumdihydrogenphosphat 1,824 g| +
-|NaCl 1-10 g| |Magnesiumchlorid 0,158 g| +
-|mit ddH20 auf 1 L||Bromthymolblau 80 mg| +
-| | |NaCl 4,806 g| +
-| | |Zitronensäure 1,489 g| +
-| | |NaOH 1,063 g| +
-| | |KOH 41 mg| +
-| | |Ammoniak ​(25%0,407 mL| +
-| | |mit ddH20 auf 1 L|+
  
- * für **Agar Platten 1,5% Agar Agar** zugeben (Volumen ca. 1/3 von Gefäß)+Lösung 3 
 +|2,8 M KAc, pH=5,1|
  
- * bei 121°C für mind. 20 min autoklavieren (bei Agar einen Rührknochen vorher hinzu geben)+Isopropanol 99% 
 + 
 +Ethanol 70 %
  
  
Line 112: Line 108:
 ===Kühlschrank (4°C):=== ===Kühlschrank (4°C):===
  
 +|WAS|WO|
 +
 +|Bakteriophagen (origin. & Lysate)| Tür links oben|
 +|Eurofin Genetics Primer| Tür rechts oben|
 +|Nippon Green Genetics|Tür Antibiotika Stock|
 +|Penicillin 5 mg/mL|Tür Antibiotika Stock|
 +|Proteinase K|Tür Antibiotika Stock|
 +|Ampicillin Natriumsaltz|Tür Antibiotika Stock|
 +|Kanamycinsulfat|Tür Antibiotika Stock|
 +|1,​4-Dithiothreit|Tür Antibiotika Stock|
 +|Geneticinsulfat|Tür Antibiotika Stock|
 +|DIY Lysozym|Tür Antibiotika Stock|
 +|diverse Agarplatten Reserve| Tür unten|
 +|
 +|Agarplatten mit Mikroorganismen| aufgeteilt im Kühlschrank|
 +
 +
 +Gefrierfach im Kühlschrank:​
 +3x Kühlelemente (BIOLabs)
 +1x blaues Kühlelement für Eppis
 +
 +
 +------------------------------
  
  
 ===Gefrierschrank (-20°C):​=== ===Gefrierschrank (-20°C):​===
 +
 +**Fach OBEN**
 +
 +      __Weiß1 = Bufferbox__
 +
 +NEBuffer2.1 ||||
 +
 +NeBuffer3.1 |
 +
 +CutSmartBuffer ||||| ||||
 +
 +OneTaqStandardReactionBuffer ||||
 +
 +OneTagGCReactionBuffer |
 +
 +OneTaqHighGCEnhancer |
 +
 +Buffer f. T4 DNA Ligase+ATP ||||
 +
 +Buffer f. T4 DNA Ligase |
 +
 +Buffer f. T7 DNA Ligase |
 +
 +TermoPolReactionBuffer |||||
 +
 +ThermoPolBuffer ||
 +
 +ATP20xStock (Ligation) |
 +
 +5x HF Puffer |
 +
 +Magnesiumchlorid
 +
 +      __Weiß2 = Primer__
 +
 +Primer reverse |
 +
 +Primer forward |
 +
 +Primer mix |
 +
 +Primer forward (Eurofins)
 +
 +Controll Template
 +
 +Controll Primer ||
 +
 +p15a forward primer
 +
 +LacZ PRimer forward |
 +
 +NDE1 reverse |
 +
 +Kpn1 forward |
 +
 +
 +
 +dNTP-SolutionMix (10mM) ||
 +
 +dNTP (2mM) |||||||||||||
 +
 +
 +
 +Lamda-DNA |
 +
 +Lamda DNA Hind3 digest |
 +
 +Quick-(1kB) DNA Ladder |
 +
 +100bp DNA Ladder |
 +
 +
 +
 +Controll Template |||
 +
 +Controll Primer |||
 +
 +
 +
 +pUC192 |
 +
 +pUC19 neu |
 +
 +pEasy T1 Cloning Vector |
 +
 +pYAC3 ||
 +
 +pGreen20049 |
 +
 +p355-GFP |
 +
 +
 +
 +CUP2 Promotor||
 +
 +SPO3 forward Primer |
 +
 +Gene Ruler 100bp |
 +
 +
 + __Grün 3 = Polymerase/​RNase__
 +
 +
 +TE + RNase |||||||||||
 +
 +Taq Polymerase |||||
 +
 +Phusion HF Dna Pol | 
 +
 +RNase-IF |
 +
 +RNase||||
 +
 +Instant Sticky Ends Ligase Master Mix |
 +
 +T4 Dna Ligase |
 +
 +T7 Dna Ligase |
 +
 +Blunt Ligation Master Mix |
 +
 +Taq Buffer
 +
 +
 +
 + __Blau4__ ​
 +
 +
 +5x Phusion GC Reaction Buffer |
 +
 +Q5 Reaction Buffer ||
 +
 +Q5 Hf Polymerase ||
 +
 +1kb + DNA Ladder |
 +
 +Eurofins Primer |
 +
 +Phusion HF Reaction Buffer |
 +
 +10mM dNTP Solution Mix |||
 +
 +Q5 High GC Enhancer ||
 +
 +OneTaq Dna Pol |
 +
 +OneTaq HotStart Buffer |
 +
 +10 kb controll Primer Mix |
 +
 +100% DMSO |
 +
 +
 + ​ __Box 5 - blauer Deckel__
 +
 +Lamda DNA |
 +
 +Buffer ​
 +
 +Eurogenomics Primer
 +
 +Primer
 +
 +LycTerm
 +
 +DNA Marker Quick Load |
 +
 +Loading Buffer
 +
 +100bP Dna Ladder
 +
 +One Taq Standard Reaction Buffer|
 +
 +
 + __Metallblock mit Restriktionsenzyme__
 +
 +Sp1 |
 +
 +Bsa!-HF ||
 +
 +Pst1 |
 +
 +Kq/pn1 | ??
 +
 +Xma1 |
 +
 +BamH1 -HF|
 +
 +Xba1 |
 +
 +Sma1|
 +
 +EcoR1-HF|
 +
 +PNK |
 +
 +Pst1 |
 +
 +T4 Dna Ligase |||
 +
 +Proteinase K |
 +
 +T4 DNA Ligase + ATP ||
 +
 +
 +
 + __Violette Box (to be used)__
 +
 +
 +Ribunuklease 100mg |
 +
 +Iptg 1g |
 +
 +HOT FIREPOL Blend Mastermix |
 +
 +xß-gal |
 +
 +Lyticase |
 +
 +GFP
 +
 +ATP 5 g
 +
 +
 +
 +
 + __Phusion High fidelity PCR Kit__
 +
 +
 +Phusion DNA Pol |
 +
 +1.3 kB Primer |
 +
 +GC Buffer|
 +
 +DMSO |
 +
 +10 kb Primer |
 +
 +Magnesiumchlorid |
 +
 +dNTP Mix |
 +
 +DNA-Marker|
 +
 +Lamda-DNA |
 +
 +
 +
 + __E.coli Selection Kit__
 +
 + ​versciedene Vektoren, Buffer, PCR-Controll,​ Restrictionsenzyme...
 +
 +
 + __STÄMME (lyophilisiert in Plastikröhrchen__
 +
 +DSM430 |
 +
 +DSM5997 |
 +
 + __Eurofins Genomix Metall__
 +
 +
 +Primer |||| 
 +
 +
 + __NAWI - iGem Plastik Hülsn__ ​
 +
 +
 +G-Blocks Gen Fragmente |
 +
 +Spezifisch-Flag-Tag |
 +
 +
 + __Transformation Efficiency Kit - Plasik Hülsn__
 +
 +
 +pSB1C3 50pg/ml - 0,5pg/ml)
 +
 +
 + __Linearisirte Plasmid Backbones - Plastik Hülsn__
 +
 +pSB1 derivate 4x 
 +
 +
 +
 +**Fach MITTE**
 +
 +Transgen Biotech - China Free Samples !!! Protokolle zu machnen Enzymen aus Fach OBEN
 +
 +
 +**Fach UNTEN**
 +
 +Unspezifische DNA (irgendwelche DNA Proben ausn Praktikum, verwenden)
 +
 +Spezifische DNA (Bekannte DNA Proben, Plasmide, usw)
 +
 +Gelladepuffer (6x)
 +
 +Antibiotika Stock Lösungen ( ready to use --> ​ für 100 mL Medium = 100 µL Stock-Lsg)
 + ​-Ampicillin
 + ​-Kanamycin
 + ​-Streptomycin
 + ​-Chloramphenicol
 +
 +Molbio Free 4 All Box (diverse Reagenzien)
 +
 +Daniel Derndorfer Box (privat Box --> nix ohne fragen herausnehmen,​ bitte :) )
 +
 +
realraum Graz, Brockmanngasse 15, 8010 Graz, realraum - Verein für Technik in Kultur und Gesellschaft
  • /var/lib/dokuwiki/data/pages/olga/olgainventarchemikalien.txt
  • Last modified: 2021-02-09 13:39
  • by danield